Furuno A, Kato M
Department of Chemistry, Faculty of Science, Shizuoka University, Japan.
Nucleic Acids Symp Ser. 1992(27):157-8.
Superhelical alteration of plasmid DNA in Escherichia coli cells was observed during cell growth. Plasmid DNAs were being supercoiled while the cells were actively dividing, and they were partially relaxed after the cultures reached stationary. Unknown structural materials of plasmid DNA which migrated faster than usual superhelices in agarose gel electrophoresis were found among the strains regardless of gyrase genotype.
在大肠杆菌细胞生长过程中观察到质粒DNA的超螺旋变化。当细胞活跃分裂时，质粒DNA呈超螺旋状态，而当培养物进入稳定期后，它们会部分松弛。无论gyrase基因型如何，在菌株中均发现了在琼脂糖凝胶电泳中迁移速度比通常的超螺旋更快的质粒DNA未知结构物质。
