Relationship between the 5'-leader sequence of mRNAs and the translation efficiency in a eukaryotic in vitro system.
作者信息
Wakiyama M, Hirao I, Kumagai I, Watanabe K, Miura K
机构信息
Institute for Biomolecular Science, Gakushuin University, Tokyo, Japan.
出版信息
Nucleic Acids Symp Ser. 1992(27):159-60.
PMID:1289802
Abstract
To examine the effect of the 5'-leader region of mRNAs in regulating translation efficiency, chimeric mRNAs which have various sequences of the 5'-leader were synthesized by the SP6 in vitro transcription system, and translated in the wheat germ in vitro translation system. Translation efficiency was affected by the length of the 5'-leader and the number of the codon A-U-G at the translation initiation site.
摘要
Zotero 插件