Beecken W-D, Jonas D
Klinik für Urologie und Kinderurologie der J.-W.-Goethe-Universität, Frankfurt am Main.
Urologe A. 2003 Jul;42(7):922-6. doi: 10.1007/s00120-003-0308-5. Epub 2003 Mar 13.
The polymerase chain reaction (PCR) is a highly sensitive and specific method for the detection of genetic material. Over the last few years, this method has been used increasingly for the molecular detection of disease. This report demonstrates the use of PCR in the diagnosis of bladder cancer. The principles of the method are shown by describing the detection of p53, CD44 and telomerase, as well as in microsatellite analysis.
聚合酶链反应(PCR)是一种用于检测遗传物质的高度灵敏且特异的方法。在过去几年中,该方法越来越多地用于疾病的分子检测。本报告展示了PCR在膀胱癌诊断中的应用。通过描述p53、CD44和端粒酶的检测以及微卫星分析,阐述了该方法的原理。