Kamiya H, Miura H, Suzuki M, Murata N, Ishikawa H, Shimizu M, Komatsu Y, Murata T, Sasaki T, Inoue H
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Nucleic Acids Symp Ser. 1992(27):179-80.
In order to investigate whether several DNA lesions (O6-methylguanine, 8-hydroxyguanine, xanthine, an abasic site analogue and hypoxanthine) activate a c-Ha-ras gene and to determine the type of mutations induced by the DNA lesions, they were introduced into a synthetic c-Ha-ras gene by DNA cassette mutagenesis techniques. The modified genes were transfected into mouse NIH3T3 cells and the c-Ha-ras genes present in transformed cells were analysed. O6-methylguanine and xanthine induced a mutation to A, hypoxanthine induced a mutation to G. 8-hydroxyguanine and the abasic site analogue caused random mutations in the modified and adjacent positions. These results indicated that the synthetic c-Ha-ras gene is very useful for the detection of mutations caused by a DNA lesion.
为了研究几种DNA损伤（O6-甲基鸟嘌呤、8-羟基鸟嘌呤、黄嘌呤、无碱基位点类似物和次黄嘌呤）是否激活c-Ha-ras基因，并确定DNA损伤诱导的突变类型，通过DNA盒式诱变技术将它们导入合成的c-Ha-ras基因中。将修饰后的基因转染到小鼠NIH3T3细胞中，并分析转化细胞中存在的c-Ha-ras基因。O6-甲基鸟嘌呤和黄嘌呤诱导突变为A，次黄嘌呤诱导突变为G。8-羟基鸟嘌呤和无碱基位点类似物在修饰位置和相邻位置引起随机突变。这些结果表明，合成的c-Ha-ras基因对于检测由DNA损伤引起的突变非常有用。
