Kamiya H, Miura K, Ishikawa H, Inoue H, Nishimura S, Ohtsuka E
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Cancer Res. 1992 Jun 15;52(12):3483-5.
To determine the type of mutation induced by 8-hydroxyguanine in a mammalian system, we examined the mutations induced by a synthetic c-Ha-ras protooncogene containing 8-hydroxyguanine in the second position of codon 12 (GGC) in NIH3T3 cells. Transfection of this gene significantly increased the number of transformed foci. The c-Ha-ras gene present in these foci was analyzed by the polymerase chain reaction-restriction enzyme method. Interestingly, sequence analysis revealed random mutations at the modified site (G----T, G----A, and G----C) as well as mutations of the adjacent G on the 5'-side of 8-hydroxyguanine (G----A and G----T).
为了确定8-羟基鸟嘌呤在哺乳动物系统中诱导的突变类型，我们检测了在NIH3T3细胞中，密码子12（GGC）第二位含有8-羟基鸟嘌呤的合成c-Ha-ras原癌基因所诱导的突变。该基因的转染显著增加了转化灶的数量。通过聚合酶链反应-限制酶法分析了这些转化灶中存在的c-Ha-ras基因。有趣的是，序列分析显示在修饰位点（G→T、G→A和G→C）出现随机突变，以及在8-羟基鸟嘌呤5'侧相邻的G发生突变（G→A和G→T）。
