Singh S, Sinha D N, Prasad G C
Acta Anat (Basel). 1975;93(3):440-6. doi: 10.1159/000144520.
Effect of mitomycin C on mucopolysaccharide synthesis by the cartilage cells of chick limb bud (5-9 days old) was investigated both in vivo and in vitro by the uptake study of 35S. Control specimens (both in vivo and in vitro) showed higher concentration of silver grains, mostly intercellular, in the initial stages. Hypertrophy of cartilage cells at later stages reduced the matrix, leading to a corresponding decrease in the accumulation of silver grains. In the treated groups, both in vivo and in vitro, the silver grains were found to be less in number during the initial stage of growth than in the corresponding controls, due to the suppressive effect of the mitomycin C. However, during the later period of growth, uptake of 35S was more in the matrix of treated specimens, which was apparently not reduced due to lack of hypertrophy of cartilage cells. The mechanism of the suppressive effect of mitomycin C seems similar in vivo and in vitro.
通过³⁵S摄取研究，在体内和体外研究了丝裂霉素C对雏鸡肢体芽（5 - 9日龄）软骨细胞黏多糖合成的影响。对照标本（体内和体外）在初始阶段显示银颗粒浓度较高，大多位于细胞间。后期软骨细胞肥大减少了基质，导致银颗粒积累相应减少。在体内和体外的处理组中，由于丝裂霉素C的抑制作用，在生长初期发现银颗粒数量比相应对照组少。然而，在生长后期，处理标本的基质中³⁵S摄取更多，这显然是由于软骨细胞未肥大而未减少。丝裂霉素C的抑制作用机制在体内和体外似乎相似。
