Corrales R M, Galarreta D J, Herreras J M, Calonge M, Chaves F J
Instituto Universitario de Oftalmobiología Aplicada (IOBA), Universidad de Valladolid, Valladolid, España.
Arch Soc Esp Oftalmol. 2003 Jul;78(7):375-81.
The ocular surface epithelia express at least five mucin genes of the total of 17 human mucin genes that have been identified so far. This study was designed to determine the expression profile of mucin genes in conjunctival impression cytology (CIC) samples from healthy subjects.
Two polyethersulfone filters were applied to the superior conjunctiva of both eyes from eight healthy donors. Polymerase chain reaction (PCR) was performed using isolated and retrotranscripted total RNA obtained from the CIC samples to study the expression of all known human mucin genes. Following amplification, PCR products were electrophoresed on 1.5% agarose gel and stained with ethidium bromide to confirm that only a single band was obtained when amplifying all cDNAs with the convenient primers.
Transcripts of the previously reported conjunctival mucin genes MUC1, MUC2, MUC4, MUC5AC, and MUC7 were detected in all samples. In addition, transcripts of MUC13, MUC15, MUC16 and MUC17 mucin genes also were detected. Amplified products by conventional PCR showed the expected amplicon size. Transcripts of MUC3A, MUC3B, MUC5B, MUC6, MUC8, MUC11, and MUC12 mucin genes were not detected.
The expression of four additional mucin mRNA (MUC13, MUC15, MUC16, and MUC17) has been proved in human conjunctival epithelium from healthy donors for the first time. The function of these genes remains to be further elucidated.
在迄今已鉴定出的17种人类黏蛋白基因中，眼表上皮至少表达其中5种。本研究旨在确定健康受试者结膜印迹细胞学（CIC）样本中黏蛋白基因的表达谱。
对8名健康供体双眼的上结膜应用两片聚醚砜滤膜。使用从CIC样本中分离并反转录的总RNA进行聚合酶链反应（PCR），以研究所有已知人类黏蛋白基因的表达。扩增后，将PCR产物在1.5%琼脂糖凝胶上进行电泳，并用溴化乙锭染色，以确认使用便捷引物扩增所有cDNA时仅获得一条带。
在所有样本中均检测到先前报道的结膜黏蛋白基因MUC1、MUC2、MUC4、MUC5AC和MUC7的转录本。此外，还检测到MUC13、MUC15、MUC16和MUC17黏蛋白基因的转录本。常规PCR扩增产物显示出预期的扩增子大小。未检测到MUC3A、MUC3B、MUC5B、MUC6、MUC8、MUC11和MUC12黏蛋白基因的转录本。
首次在健康供体的人结膜上皮中证实了另外四种黏蛋白mRNA（MUC13、MUC15、MUC16和MUC17）的表达。这些基因的功能仍有待进一步阐明。
