Machowicz-Matejko Eulalia, Łańcut MirosŁaw, Jedrych Barbara, Zakrzewski Paweł, Czerny Krystyna
Department of Histology and Embryology, Medical University of Lublin.
Ann Univ Mariae Curie Sklodowska Med. 2002;57(1):61-6.
The aim of this study was to observe in electron microscope the New Zealand rabbits ciliary epithelial nucleus structure changes, depending on the duration of experimental diabetes mellitus. The animals were administered alloxan in the form of 10% solution in 0.9% NaCl, at the dose of 100 mg/kg b.m. If the glycemia level was 11 mmol/l or higher the animal was included in the experimental group. 15 eyes of 15 adult New Zealand rabbits were studied. Conventional electron microscopy was used to show ultrastructural changes of ciliary epithelium nucleus. During the study we noticed severe changes in the structure of ciliary epithelial nucleus depending on diabetes mellitus duration. We found: deep indentations of nuclear membrane, open nuclear pores, irregularity in heterochromatin location and small sizes of the epithelial nucleus.
本研究的目的是通过电子显微镜观察新西兰兔睫状上皮细胞核结构的变化，该变化取决于实验性糖尿病的持续时间。以10%的溶液形式在0.9%的氯化钠中给动物注射四氧嘧啶，剂量为100mg/kg体重。如果血糖水平为11mmol/l或更高，则将该动物纳入实验组。对15只成年新西兰兔的15只眼睛进行了研究。采用常规电子显微镜观察睫状上皮细胞核的超微结构变化。在研究过程中，我们注意到睫状上皮细胞核的结构根据糖尿病持续时间发生了严重变化。我们发现：核膜深度凹陷、核孔开放、异染色质位置不规则以及上皮细胞核体积变小。
