Desai M R, Ghosh S K
Microbiology Division, National Institute of Occupational Health, Meghani Nagar, Ahmedabad 380 016, India.
Cell Mol Biol (Noisy-le-grand). 2003 Jun;49(4):529-35.
A study was undertaken on environmental mycoflora of a maize processing industry in Ahmedabad. The airborne fungal communities were isolated and identified both qualitatively by Petri-plate exposure method and quantitatively by using Andersen-6-stage viable sampler, Midget impinger and high volume samples (cone and Hexhlet for total and respirable dusts, respectively). Of all the isolates genus Aspergillus was the dominant environmental mycoflora and among all the species of Aspergillus A. flavus was the common isolates irrespective of the method applied for sample collection. Maximum number of isolates were recovered from Elevator department. From total and respirable dusts, about 56.6% and 44.4% of recovery accounted for genus Aspergillus alone. Total percentages of aflatoxin positive strains of A. flavus were 5.65% and 9.73% from total and respirable dusts, respectively. These toxigenic strains were identified on various media like CZ with 0.05% anisaldehyde, APA and CAM. Surface morphology of toxigenic strains and dust samples were carried out using SEM.
对艾哈迈达巴德一家玉米加工业的环境真菌区系进行了一项研究。通过平板暴露法对空气中的真菌群落进行定性分离和鉴定，并使用安德森六级活菌采样器、小型冲击器和大容量采样器（分别用于采集总粉尘和可吸入粉尘的锥形采样器和赫克斯利特采样器）进行定量分析。在所有分离菌株中，曲霉属是主要的环境真菌区系，在所有曲霉属物种中，无论采用何种采样方法，黄曲霉都是常见的分离菌株。从电梯部门回收的分离菌株数量最多。仅曲霉属就分别占总粉尘和可吸入粉尘回收率的约56.6%和44.4%。黄曲霉产黄曲霉毒素阳性菌株在总粉尘和可吸入粉尘中的总百分比分别为5.65%和9.73%。这些产毒菌株在多种培养基上进行鉴定，如含0.05%茴香醛的CZ培养基、APA培养基和CAM培养基。使用扫描电子显微镜对产毒菌株和粉尘样本的表面形态进行了观察。
