Ji Kai-yu, Hu Fu-jian, Li Ai-dong, Li Jiang
Department of Gastroenterology, Peking University Hospital, Beijing 100034, China.
Zhonghua Yi Xue Za Zhi. 2003 May 10;83(9):726-30.
To investigate the interaction of Helicobacter pylori (Hp) and indomethacin in gastric mucosa injury of Balb/c mice.
Fifty specific pathogen free 6-week-old Balb/c mice were randomly divided into 5 groups: group A (n = 8, 25 mg/kg indomethacin was injected intraperitoneally every other day for 6 times, and 0.2 ml Hp culture containing 1 x 10(8) - 1 x 10(9) CFU/ml was inoculated intra-gastrically every other day for 6 times), B (n = 8, 1 x 10(8) - 1 x 10(9) CFU/ml Hp culture was inoculated intra-gastrically on the 1st, 2nd, and 3rd days, and since the 7th day 25 mg/kg indomethacin was injected intraperitoneally every other day for 6 tomes), group C (n = 9, 0.2 ml normal saline was inoculated intra-gastrically on the 1st, 2nd, and 3rd days and since the 7th day 25 mg/kg indomethacin was injected intraperitoneally every other day for 6 times), group D (n = 10, 1 x 10(8) - 1 x 10(9) CFU/ml Hp culture was inoculated intra-gastrically on the 1st, 2nd, and 3rd days, and since the 7th day 0.2 ml normal saline was injected intraperitoneally every other day for 6 times), and group E (n = 10, 0.2 ml normal saline was inoculated intra-gastrically on the 1st, 2nd, and 3rd days, then 0.2 ml normal saline was injected intraperitoneally since the 7th day every other day for 6 times). The mice were killed in the 4th week. The stomachs were observed morphologically. Tissues from gastric antrum underwent HE staining, silver staining, and urease examination. Rauws classification was used to grade the gastritis thus discovered.
Two mice in group A, one mouse in group B and 2 mice in group C died. One mouse in group B was Hp silver staining negative. Twenty-six of the total 30 mice infected with Hp showed inflammation with an infection rate of 66.7%. Moderate to severe inflammatory reaction was seen in all 8 mice in group A with an average gastritis score of 6.38 and with Hp found in the epithelium. Two out of the 8 mice in group showed focal erosion. All mice in group B showed moderate inflammation and Hp in mucosa with an average gastritis score of 5.13. Focal erosion was seen in one mouse in group B. Light moderate inflammation was seen in all 9 mice in group C with an average gastritis score of 3.89. Mild inflammation was seen in all 10 mice in group D with an average gastritis score of 2.8. No erosion was found in groups C and D. No inflammation was seen in the mice in group E with an average gastritis score of 0.4. The differences between any 2 groups were all significant.
Hp and indomethacin synergetically aggravate the gastric epithelial injury. Prior Hp infection may be a protective factor of indomethacin-induced gastritis to a certain degree.
探讨幽门螺杆菌（Hp）与吲哚美辛在Balb/c小鼠胃黏膜损伤中的相互作用。
将50只6周龄无特定病原体的Balb/c小鼠随机分为5组：A组（n = 8，每隔一天腹腔注射25 mg/kg吲哚美辛，共6次，每隔一天经胃接种含1×10⁸ - 1×10⁹ CFU/ml的Hp培养物0.2 ml，共6次），B组（n = 8，第1、2、3天经胃接种1×10⁸ - 1×10⁹ CFU/ml的Hp培养物，自第7天起每隔一天腹腔注射25 mg/kg吲哚美辛，共6次），C组（n = 9，第1、2、3天经胃接种0.2 ml生理盐水，自第7天起每隔一天腹腔注射25 mg/kg吲哚美辛，共6次），D组（n = 10，第1、2、3天经胃接种1×10⁸ - 1×10⁹ CFU/ml的Hp培养物，自第7天起每隔一天腹腔注射0.2 ml生理盐水，共6次），E组（n = 10，第1、2、3天经胃接种0.2 ml生理盐水，自第7天起每隔一天腹腔注射0.2 ml生理盐水，共6次）。第4周处死小鼠，观察胃的形态。取胃窦组织进行HE染色、银染色和尿素酶检查。采用Rauws分类法对所发现的胃炎进行分级。
A组2只小鼠、B组1只小鼠和C组2只小鼠死亡。B组1只小鼠Hp银染色阴性。30只感染Hp的小鼠中26只出现炎症，感染率为66.7%。A组8只小鼠均出现中度至重度炎症反应，平均胃炎评分为6.38，上皮细胞中发现Hp。B组8只小鼠中有2只出现局灶性糜烂。B组所有小鼠均表现为中度炎症，黏膜中有Hp，平均胃炎评分为5.13。B组1只小鼠出现局灶性糜烂。C组9只小鼠均出现轻度至中度炎症，平均胃炎评分为3.89。D组10只小鼠均出现轻度炎症，平均胃炎评分为2.8。C组和D组均未发现糜烂。E组小鼠未出现炎症，平均胃炎评分为0.4。任意两组之间的差异均有统计学意义。
Hp与吲哚美辛协同加重胃上皮损伤。Hp预先感染在一定程度上可能是吲哚美辛诱导胃炎的一个保护因素。
