Gao Wen, Hu Fu-lian, Lü You-yong
Department of Gastroenterology, First Hospital of Peking University, Beijing 100034, China.
Zhonghua Yi Xue Za Zhi. 2003 May 10;83(9):731-5.
To observe the effects of Helicobacter pylori (H. pylori) on growth and cell cycle progression of AGS gastric epithelial cells.
AGS cells and H. pylori were co-cultured for 1 - 4 days. Vital cell numbers were counted by heamatocounter. Apoptosis was detected by fluorescence stain of cell nucleus and DNA agarose electrophoresis. Cell cycle was analyzed by flow cytometry technique.
During co-culture of AGS cells and H. pylori, cell growth inhibition was observed. H. pylori induces apoptosis and cell cycle arrest from G1 to S phase accompanied by high expression of cyclinD1.
Exposure of gastric epithelial cells to H. pylori causes block of G1 to S progression of cell cycle and alters expression of cyclinD1. The cell cycle arrest and the associated induction of apoptosis are responsible for the growth inhibition of gastric epithelial cells caused by H. pylori.
观察幽门螺杆菌（H. pylori）对AGS胃上皮细胞生长和细胞周期进程的影响。
将AGS细胞与幽门螺杆菌共培养1 - 4天。用血细胞计数器计数活细胞数量。通过细胞核荧光染色和DNA琼脂糖电泳检测细胞凋亡。采用流式细胞术分析细胞周期。
在AGS细胞与幽门螺杆菌共培养期间，观察到细胞生长受到抑制。幽门螺杆菌诱导细胞凋亡并使细胞周期从G1期阻滞到S期，同时伴有细胞周期蛋白D1的高表达。
胃上皮细胞暴露于幽门螺杆菌会导致细胞周期从G1期到S期的进程受阻，并改变细胞周期蛋白D1的表达。细胞周期阻滞及相关的细胞凋亡诱导是幽门螺杆菌导致胃上皮细胞生长抑制的原因。
