Wang Wen-ling, Li Ruo-yu, Zhu Xue-jun, Wang Duan-li
Department of Dermatology, Peking University First Hospital, Research Center for Medical Mycology, Peking University, Beijing 100034, China.
Zhonghua Yi Xue Za Zhi. 2003 May 10;83(9):787-90.
To screen out the differential expression genes between the immune suppressive host and healthy host after intranasal inoculation of Aspergillus fumigatus (A fumigatus).
Six immune suppressive mice and 6 healthy mice were inoculated with A, fumigatus intranasally. Three days after the inoculation the mice were killed and autopsied. The total RNA of the lungs was isolated. Gene chip technology was used to examine the differentially expressed genes.
66 differentially expressed genes were screened out of the 4098 genes. In the experimental group the genes encoding CD22, CD53, and 24p3 and the genes related to engulfment and digestion of macrophage or monocytes were up-regulated. The genes encoding surfactant associated protein C, immunoglobulin lambda chain and the genes related to pulmonary metabolism were down-regulated. The genes encoding IL18, CD8, and CD28 were not differentially expressed.
The up-regulation of CD22 gene may be one of the ways by which the host defense is inhibited. CD53 may take part in the regulation of engulfment of phagocytes. 24p3 protein, an acute stress protein, protects the host from excessive inflammatory response.
筛选出经鼻接种烟曲霉后免疫抑制宿主与健康宿主之间的差异表达基因。
对6只免疫抑制小鼠和6只健康小鼠经鼻接种烟曲霉。接种3天后处死小鼠并进行尸检。分离肺组织的总RNA。采用基因芯片技术检测差异表达基因。
在4098个基因中筛选出66个差异表达基因。实验组中，编码CD22、CD53和24p3的基因以及与巨噬细胞或单核细胞吞噬和消化相关的基因上调。编码表面活性物质相关蛋白C、免疫球蛋白λ链的基因以及与肺代谢相关的基因下调。编码IL18、CD8和CD28的基因无差异表达。
CD22基因上调可能是宿主防御受到抑制的途径之一。CD53可能参与调节吞噬细胞的吞噬作用。24p3蛋白作为一种急性应激蛋白，可保护宿主免受过度炎症反应的影响。
