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使用超氧化物歧化酶生物传感器测定不同类型茶叶样品、基于茶叶或其他草药产品的饮品的抗氧化能力。

Determination of the antioxidant capacity of samples of different types of tea, or of beverages based on tea or other herbal products, using a superoxide dismutase biosensor.

作者信息

Campanella L, Bonanni A, Tomassetti M

机构信息

Department of Chemistry, University of Rome La Sapienza, Piazza Aldo Moro, 5, 00185 Rome, Italy.

出版信息

J Pharm Biomed Anal. 2003 Aug 8;32(4-5):725-36. doi: 10.1016/s0731-7085(03)00180-8.

Abstract

Research was performed to experimentally evaluate the antioxidant capacity of different plant products sold by herbalists (ginger, dog rose, ginseng and camomile) and of several types of tea (ordinary tea, green tea, detheinated tea, lemon and peach flavoured tea) using a superoxide dismutase (SOD) biosensor recently developed by the present authors. Measurements were carried out by comparing biosensor response to the superoxide radical produced in solution using the xanthine-xanthine oxidase system, both in the presence and absence of the antioxidant sample considered. Precision of antioxidant capacity measures for herbal products and for non diluted samples was good, generally with a R.S.D.%< or =10% and a LOD value about 0.1 for relative antioxidant capacity. Also a "pool" of polyphenols from different tea samples was measured using a tyrosinase biosensor (LOD approximately 2 microM).

摘要

研究采用作者最近开发的超氧化物歧化酶(SOD)生物传感器,对草药商出售的不同植物产品(生姜、狗蔷薇、人参和洋甘菊)以及几种茶(普通茶、绿茶、脱咖啡因茶、柠檬和桃子味茶)的抗氧化能力进行了实验评估。通过比较生物传感器对使用黄嘌呤-黄嘌呤氧化酶系统在溶液中产生的超氧阴离子自由基的响应来进行测量,测量时既有抗氧化剂样品存在的情况,也有不存在该样品的情况。草药产品和未稀释样品抗氧化能力测量的精密度良好,相对抗氧化能力的R.S.D.%通常≤10%,检测限约为0.1。还使用酪氨酸酶生物传感器对不同茶样品中的多酚“库”进行了测量(检测限约为2 microM)。

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