Aboul-Enein Hassan Y, El-Awady Mahmoud I, Heard Charles M
Pharmaceutical Analysis Laboratory, Biological and Medical Research Department (MBC-03-65), King Faisal Specialist Hospital and Research Centre, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
J Pharm Biomed Anal. 2003 Aug 8;32(4-5):1055-9. doi: 10.1016/s0731-7085(03)00208-5.
The enantiomeric resolution of certain 2-arylpropionic acids was achieved on thin silica gel plates impregnated with optically pure L-(-)-serine as chiral selector. The mobile phase enabling successful resolution of (+/-)-ibuproxam and (+/-)-ketoprofen was acetonitrile-methanol-water (16:4:0.5, v/v/v) and (16:3:0.5, v/v/v) for (+/-)-tiaprofenic acid. The spots were detected with iodine vapors and the detection limits were found to be different for each of the 2-arylpropionic acid, ranging between 0.25 and 0.50 microg/ml. The effect of concentration of the impregnating chiral selector, temperature and pH on resolution has been studied. The procedure was applied successfully to resolve commercial ampoules of ketoprofen dosage formulation.
在浸渍有光学纯L-(-)-丝氨酸作为手性选择剂的硅胶薄板上实现了某些2-芳基丙酸的对映体拆分。能够成功拆分(+/-)-布洛芬和(+/-)-酮洛芬的流动相是乙腈-甲醇-水（16:4:0.5，v/v/v），而对于(+/-)-噻洛芬酸，流动相是乙腈-甲醇-水（16:3:0.5，v/v/v）。用碘蒸气检测斑点，发现每种2-芳基丙酸的检测限不同，范围在0.25至0.50微克/毫升之间。研究了浸渍手性选择剂的浓度、温度和pH对拆分的影响。该方法成功应用于拆分酮洛芬剂型的市售安瓿。
