Bhushan R, Thiongo G T
Department of Chemistry, University of Roorkee, India.
Biomed Chromatogr. 1999 Jun;13(4):276-8. doi: 10.1002/(SICI)1099-0801(199906)13:4<276::AID-BMC843>3.0.CO;2-M.
Direct enantioseparation of (+/-)-ibuprofen and (+/-)-flurbiprofen was achieved by two-dimensional thin-layer chromatography on silica gel plates impregnated with optically pure (-)-brucine as chiral selector. The solvent systems that were successful in resolving both the compounds were acetonitrile-methanol (16:3 v/v) for the first dimension and acetonitrile-methanol-water (16:3:0.4, v/v) for the second dimension. Iodine vapour was used for detection; the detection limit for both (+/-)-ibuprofen and (+/-)-flurbiprofen was 0.1 microgram.
通过在浸渍有光学纯（-）-马钱子碱作为手性选择剂的硅胶板上进行二维薄层色谱法，实现了（±）-布洛芬和（±）-氟比洛芬的直接对映体分离。成功分离这两种化合物的溶剂系统，第一维为乙腈-甲醇（16:3 v/v），第二维为乙腈-甲醇-水（16:3:0.4，v/v）。使用碘蒸气进行检测；（±）-布洛芬和（±）-氟比洛芬的检测限均为0.1微克。
