Sapunova L I, Lobanok A G, Parakhnia E V, Kazakevich I O
Institute of Microbiology, National Academy of Sciences of Belarus, ul. Kuprevicha 2, Minsk, 220141 Belarus.
Mikrobiologiia. 2003 May-Jun;72(3):395-9.
The study of the xylose/glucose isomerase-containing Arthrobacter sp. B-5 cells immobilized in cobalt hydroxide gel showed that immobilization increases the substrate affinity of the enzyme, its thermo- and pH-optima of action and stability, and makes unnecessary the addition of stabilizing cobalt ions to the isomerization medium.
对固定在氢氧化钴凝胶中的含木糖/葡萄糖异构酶的节杆菌属B-5细胞的研究表明,固定化提高了酶的底物亲和力、其作用的温度和pH最佳值以及稳定性,并且无需向异构化介质中添加稳定的钴离子。