Hu Y S, Zhang Q L, Tian Z G, Wei H M, Li G, Pang Q, Feng J B, Xu X Q, Wang C W, Sun R
Department of Neurosurgery, Second Hospital, Shandong University, Jinan 250033, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001 Dec;23(6):594-8.
To study the significance of the unbalanced expression of Th1/Th2 type cytokines in human glioma.
The gene expressions of Th1/Th2 type cytokines in 62 specimens of human glioma tissues, 4 glioma cell lines, peripheral blood mononuclear cell (PBMC) of 15 glioma patients, 5 specimens of normal adult brain tissue and 5 brain meningioma tissues were detected by semiquantitative reverse transcription polymerase chain reaction. IFN-gamma and IL-2 represent Th1 type cytokines. IL-4, IL-6, IL-10 and IL-13 represent Th2 type cytokines.
There were obviously predominant expression of Th2 type cytokines in glioma cell lines (P < 0.01) and specimens of human glioma tissues (P < 0.01). The tendency of distinct expression of Th2 type cytokines in PBMC was also existent. There wasn't obvious discrepancy of the expression of two type cytokines in normal adult brain tissues and meningioma tissues.
It is likely that the switching of Th1/Th2 type cytokines in gliomas as predominant expression of Th2 type cytokine genes is related to the origination of gliomas and the evasion of glioma cells from immune surveillance.
研究Th1/Th2型细胞因子失衡表达在人类胶质瘤中的意义。
采用半定量逆转录聚合酶链反应检测62例人类胶质瘤组织标本、4种胶质瘤细胞系、15例胶质瘤患者外周血单个核细胞（PBMC）、5例正常成人脑组织标本和5例脑膜瘤组织中Th1/Th2型细胞因子的基因表达。IFN-γ和IL-2代表Th1型细胞因子。IL-4、IL-6、IL-10和IL-13代表Th2型细胞因子。
Th2型细胞因子在胶质瘤细胞系（P < 0.01）和人类胶质瘤组织标本（P < 0.01）中均有明显的优势表达。PBMC中Th2型细胞因子的明显表达趋势也存在。正常成人脑组织和脑膜瘤组织中两种类型细胞因子的表达无明显差异。
胶质瘤中Th1/Th2型细胞因子的转换以Th2型细胞因子基因的优势表达为特征，这可能与胶质瘤的发生及胶质瘤细胞逃避免疫监视有关。
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