Wei Yu-cheng, Hu Feng-biao, Shen Yi, Xu Lin-hao, Lin Le-sheng
Department of Thoracic Surgery, Affiliated Hospital, Medical College of Qingdao University, Qingdao 266003, China.
Di Yi Jun Yi Da Xue Xue Bao. 2004 Nov;24(11):1271-3.
To study the expressions of Th1 and Th2 type cytokines in patients with esophageal squamous cell carcinoma and the influence of operation on such expressions.
Enzyme-linked immunosorbent assay (ELISA) was used to detect Th1 (IL-2 and INF-gamma) and Th2 type cytokines (IL-4, IL-6 and IL-10) in cultured peripheral blood mononuclear cells (PBMCs, for 48 h) from 30 healthy controls and 46 esophageal squamous cell carcinoma patients (collected before and 8 and 30 d after operation, respectively).
Compared with the control group, the expressions of Th1 type cytokines, IL-2 and INF-gamma, were significantly depressed in the PBMCs of the cancer patients (P<0.05), while expression of Th2 type cytokines, IL-6 was significantly enhanced (P<0.05), and IL-4 and IL-10 showed only slight enhancement. Such changes in the expressions of the cytokines showed a correlation with TNM (tumor-node-metastasis)stage of the tumors. After operation, the expressions of Th1 type cytokines increased and Th2 type cytokines decreased.
Th1 cells are suppressed in patients with esophageal squamous cell carcinoma, while the function of Th2 cells is enhanced. Operation may reverse such changes, and dynamic detection of Th1 and Th2 type cytokines may indicate the prognosis, therapeutic effect and risks of relapse and metastasis.
研究食管鳞状细胞癌患者Th1和Th2型细胞因子的表达情况以及手术对此类表达的影响。
采用酶联免疫吸附测定法（ELISA）检测30例健康对照者及46例食管鳞状细胞癌患者（分别于术前、术后8天和30天采集）培养48小时的外周血单个核细胞（PBMCs）中Th1（IL-2和INF-γ）和Th2型细胞因子（IL-4、IL-6和IL-10）。
与对照组相比，癌症患者PBMCs中Th1型细胞因子IL-2和INF-γ的表达显著降低（P<0.05），而Th2型细胞因子IL-6的表达显著增强（P<0.05），IL-4和IL-10仅略有增强。细胞因子表达的此类变化与肿瘤的TNM（肿瘤-淋巴结-转移）分期相关。术后，Th1型细胞因子表达增加，Th2型细胞因子表达降低。
食管鳞状细胞癌患者Th1细胞受到抑制，而Th2细胞功能增强。手术可能逆转此类变化，动态检测Th1和Th2型细胞因子可能预示预后、治疗效果以及复发和转移风险。
