Zhao Y, Qiu G X
Department of Orthopedic Surgery, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001 Dec;23(6):619-22.
To determine the relationship between angiogenesis and vascular endothelial growth factor (VEGF) expression in osteosarcoma.
The expression of VEGF was evaluated by immunohistochemical staining of tumor specimens from 33 patients with osteosarcoma. The microvessel density was assessed by immunostaining with CD34 in the most neovascularized area (hot spots).
In osteosarcoma, the mean microvessel count of VEGF strong-expression tumors was significantly higher than that of VEGF low-expression tumors (Wilcoxon rank sum test, P < 0.05). The close correlation was found between VEGF expression and microvessel density. In contrast, no association was found between Price's grade of osteosarcoma and VEGF expression, as well as microvessel density.
These observations suggest that VEGF is an important angiogenic factor in osteosarcoma.
确定骨肉瘤中血管生成与血管内皮生长因子（VEGF）表达之间的关系。
通过对33例骨肉瘤患者肿瘤标本进行免疫组织化学染色来评估VEGF的表达。在血管生成最活跃的区域（热点区）用CD34免疫染色评估微血管密度。
在骨肉瘤中，VEGF强表达肿瘤的平均微血管计数显著高于VEGF低表达肿瘤（Wilcoxon秩和检验，P<0.05）。发现VEGF表达与微血管密度之间密切相关。相反，未发现骨肉瘤的普赖斯分级与VEGF表达以及微血管密度之间存在关联。
这些观察结果表明VEGF是骨肉瘤中一种重要的血管生成因子。
