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使用显色分离培养基检测食品中的单核细胞增生李斯特菌。

Detection of listeria monocytogenes in foodstuffs using chromogenic isolation media.

作者信息

Sacchetti R, Bianucci F, Ambrogiani E

机构信息

Department of Medicine and Public Health, Division of Hygiene, University of Bologna, Italy.

出版信息

New Microbiol. 2003 Jul;26(3):269-74.

Abstract

A study was carried out to compare two selective plating media commonly used to detect Listeria monocytogenes in food samples (Palcam and Oxford agar) with two more recently formulated chromogenic media (ALOA and Rapid'L.mono). A comparison was also made between the identification systems API-Listeria and Monocytogenes ID Discs. The results obtained from the analysis of 132 food samples showed that the chromogenic media allowed Listeria monocytogenes to be detected more rapidly and with higher levels of specificity and sensitivity. The efficiency of the Monocytogenes ID Discs test was confirmed, giving results comparable to those obtained with API-Listeria, but in a simpler and quicker manner.

摘要

开展了一项研究,比较常用于检测食品样本中单核细胞增生李斯特菌的两种选择性平板培养基(Palcam和牛津琼脂)与两种最新配制的显色培养基(ALOA和Rapid'L.mono)。还对鉴定系统API-Listeria和单核细胞增生李斯特菌鉴定盘进行了比较。对132份食品样本的分析结果表明,显色培养基能够更快速地检测出单核细胞增生李斯特菌,且特异性和灵敏度更高。单核细胞增生李斯特菌鉴定盘检测的效率得到了证实,其结果与API-Listeria相当,但方式更简单、更快捷。

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