一种用于从海洋环境中分离单核细胞增生李斯特菌的新型显色平板培养基的性能

Performance of a new chromogenic plating medium for the isolation of Listeria monocytogenes from marine environments.

作者信息

El Marrakchi A, Boum'handi N, Hamama A

机构信息

Institut Agronomique et Vétérinaire Hassan II, Rabat-Instituts, Rabat, Morocco.

出版信息

Lett Appl Microbiol. 2005;40(2):87-91. doi: 10.1111/j.1472-765X.2004.01651.x.

DOI:10.1111/j.1472-765X.2004.01651.x

PMID:15644105

Abstract

AIMS

This study investigated the performance of a new chromogenic plating medium for the detection of Listeria monocytogenes from naturally contaminated samples obtained from marine environments in Morocco in comparison with the conventional plating media PALCAM and Oxford.

METHODS

A total of 479 marine samples (sea water, sediment and mussels) were collected from 16 littoral sites in the region of Agadir (western centre of Morocco). They were examined for the presence of L. monocytogenes using a slight modification of the standardized French method (AFNOR V 08-055) for the detection of L. monocytogenes from food and three different isolation media: PALCAM, Oxford and a new chromogenic plating medium.

RESULTS AND SIGNIFICANCE OF THE STUDY

The Oxford and the new chromogenic plating media were found relatively more efficient than the PALCAM medium for the isolation of L. monocytogenes (chi-square test, P < 0.05) from marine samples. However, the new chromogenic plating medium was significantly more selective for L. monocytogenes (P < 0.005) than the two other isolation media as 87.5% of the suspect colonies on this medium were indeed confirmed through identification of the isolates vs 12.7% for Oxford and only 3.8% for the PALCAM medium.

摘要

目的

本研究调查了一种新型显色平板培养基在检测从摩洛哥海洋环境中获取的天然污染样本中的单核细胞增生李斯特菌时的性能，并与传统平板培养基PALCAM和牛津培养基进行比较。

方法

从阿加迪尔地区（摩洛哥西部中心）的16个沿海地点采集了总共479份海洋样本（海水、沉积物和贻贝）。使用对检测食品中单核细胞增生李斯特菌的标准化法国方法（AFNOR V 08 - 055）进行了轻微修改，并采用三种不同的分离培养基：PALCAM、牛津培养基和一种新型显色平板培养基，对样本中单核细胞增生李斯特菌的存在情况进行检测。

研究结果及意义

在从海洋样本中分离单核细胞增生李斯特菌方面，发现牛津培养基和新型显色平板培养基比PALCAM培养基相对更有效（卡方检验，P < 0.05）。然而，新型显色平板培养基对单核细胞增生李斯特菌的选择性明显高于其他两种分离培养基（P < 0.005），因为该培养基上87.5%的可疑菌落通过分离株鉴定得到了确认，而牛津培养基为12.7%，PALCAM培养基仅为3.8%。