胃癌中DCC和APC/MCC基因位点杂合性缺失的研究。
Study of loss of heterozygosity at DCC and APC/MCC genetic loci of gastric cancer.
作者信息
Wang D, Fang D, Luo Y, Lu R, Liu W
机构信息
Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038.
出版信息
Chin Med Sci J. 1999 Jun;14(2):107-11.
In order to assess the role of the changes of DCC and APC/MCC genes in the development and progression of gastric cancer, the loss of heterozygosity (LOH) of these genetic loci was investigated in 45 surgical specimens of gastric cancer with PCR-RFLP. The rate of LOH was 30.0% (9/30) at APC/MCC gene and 33.3% (15/45) at DCC gene. LOH was found in both intestinal and gastric types of gastric cancer and the rate of LOH of DCC gene was significantly higher in stages III to approximately IV gastric cancer (48.0%) than in stages I to approximately II (15.0%) (P<0.05). LOH of APC/MCC gene could be found in both early and advanced stages of gastric cancer. These findings suggest that changes of DCC and APC/MCC genes are involved in the development and progression of the intestinal and gastric types of gastric cancer.
为了评估DCC和APC/MCC基因变化在胃癌发生发展过程中的作用，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，对45例胃癌手术标本中这些基因位点的杂合性缺失（LOH）情况进行了研究。APC/MCC基因的LOH发生率为30.0%（9/30），DCC基因的LOH发生率为33.3%（15/45）。在肠型和胃型胃癌中均发现了LOH，且DCC基因在Ⅲ期至Ⅳ期胃癌中的LOH发生率（48.0%）显著高于Ⅰ期至Ⅱ期（15.0%）（P<0.05）。APC/MCC基因的LOH在胃癌的早期和晚期均可发现。这些结果提示，DCC和APC/MCC基因的变化参与了肠型和胃型胃癌的发生发展过程。
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