C型利钠肽抑制大鼠血管内皮损伤后血管平滑肌中α1-肾上腺素能受体和肌醇1,4,5-三磷酸受体的上调。

C-type natriuretic peptide inhibits upregulation of alpha1-adrenoceptor and inositol 1,4,5-trisphosphate receptor in rat vascular smooth muscle after vascular endothelial injury.

作者信息

Xiaohong W, Jun Y, Lijia T, Jingyi S, Chaoshu T, Naikui L

机构信息

Institute of Cardiovascular Research, the First Hospital, Beijing Medical University, Beijing 100034.

出版信息

Chin Med Sci J. 2000 Jun;15(2):73-8.

PMID:12901627

Abstract

OBJECTIVE

In a model of balloon injury of rat aortic endothelium, the effects of C-type natriuretic peptide (CNP) on alpha1-adrenoreceptor and inositol 1,4,5-triphosphate (IP3) receptor were studied.

METHODS

Aortic injuries were produced by vascular endothelium-denudation. Alpha1-adrenoreceptor in smooth muscle sarcolemma and IP3 receptor in smooth muscle sarcoplasmic reticulum in the rat aorta were assayed by radioactive analysis method.

RESULTS

It was found that neointima was formed and the contents of DNA, collagen and elastin of each intima-media were sigificantly increased in 7 days and 21 days after balloon injury of rat aorta. Alpha1-adrenoreceptor in smooth muscle sarcolemma and IF3 receptor in sarcoplasmic reticulum were also upregulated. Results also showed that the administration of CNP i.p. significantly decreased the contents of DNA, collagen and elastin of each intima-media, and inhibited the up-regulation of alpha1-adrenoreceptor and IP3 receptor.

CONCLUSION

The inhibition of the up-regulation of alpha1-adrenoreceptor and IP3 receptor by CNP might be one of the mechanisms of its suppressive action on intimal proliferation.

摘要

目的

在大鼠主动脉内皮球囊损伤模型中，研究C型利钠肽（CNP）对α1-肾上腺素能受体和肌醇1,4,5-三磷酸（IP3）受体的影响。

方法

通过血管内皮剥脱制作主动脉损伤模型。采用放射性分析法检测大鼠主动脉平滑肌肌膜中的α1-肾上腺素能受体和平滑肌肌浆网中的IP3受体。

结果

发现大鼠主动脉球囊损伤后7天和21天，有新生内膜形成，各内膜-中膜的DNA、胶原蛋白和弹性蛋白含量显著增加。平滑肌肌膜中的α1-肾上腺素能受体和肌浆网中的IP3受体也上调。结果还显示，腹腔注射CNP可显著降低各内膜-中膜的DNA、胶原蛋白和弹性蛋白含量，并抑制α1-肾上腺素能受体和IP3受体的上调。