印度嚼烟者口腔癌和白斑组织中APC和MCC基因杂合性缺失
Loss of heterozygosity at APC and MCC genes of oral cancer and leukoplakia tissues from Indian tobacco chewers.
作者信息
Sikdar Nilabja, Paul Ranjan Rashmi, Panda Chinmay K, Banerjee Sanat K, Roy Bidyut
机构信息
Anthropology and Human Genetics Unit, Biological Sciences Division, Indian Statistical Institute, 203 B. T. Road, Kolkata 700 08, India.
出版信息
J Oral Pathol Med. 2003 Sep;32(8):450-4. doi: 10.1034/j.1600-0714.2003.00132.x.
BACKGROUND
Loss of heterozygosity (LOH) at tumor suppressor genes, such as adenomatous polyposis coli (APC) and mutated in colon cancer (MCC) genes, is one of the early events in carcinogenesis of oral tissue in Caucasian and Chinese patients. We wanted to check whether it is also true in Indian oral pre-cancer and cancer patients.
METHODS
Loss of heterozygosity at APC and MCC genes was investigated in 57 and 40 unrelated primary oral leukoplakia (a pre-cancerous lesion) and squamous cell carcinomas (SCC), respectively, by polymerase chain reaction.
RESULTS
In these samples, most of the leukoplakia patients had tobacco smoking habit whereas majority of cancer patients had tobacco chewing habit. LOH at APC gene was observed in 4 of 16 (25%) and 1 of 29 (3%) informative tumor and leukoplakia DNAs from tobacco chewers, respectively. LOH at MCC gene was not detected either in tumor or in leukoplakia DNAs.
CONCLUSION
This infrequent LOH at APC gene of pre-cancer and cancer tissues suggests that it may not be an early event in oral carcinogenesis in these patients.
背景
肿瘤抑制基因杂合性缺失（LOH），如腺瘤性息肉病基因（APC）和结肠癌突变基因（MCC），是白种人和中国患者口腔组织癌变的早期事件之一。我们想检查这在印度口腔癌前病变和癌症患者中是否也成立。
方法
通过聚合酶链反应分别对57例和40例无关的原发性口腔白斑（一种癌前病变）和鳞状细胞癌（SCC）患者的APC和MCC基因杂合性缺失进行研究。
结果
在这些样本中，大多数白斑患者有吸烟习惯，而大多数癌症患者有咀嚼烟草的习惯。在咀嚼烟草者的16份信息性肿瘤DNA和29份白斑DNA中，分别有4份（25%）和1份（3%）检测到APC基因杂合性缺失。在肿瘤或白斑DNA中均未检测到MCC基因杂合性缺失。
结论
癌前和癌组织中APC基因这种罕见的杂合性缺失表明，这可能不是这些患者口腔癌变的早期事件。
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