Mukherjee Sanjit, Ray Jay Gopal, Chaudhuri Keya
Molecular and Human Genetics Division, Indian Institute of Chemical Biology, Kolkata, India.
Indian J Dent Res. 2011 Sep-Oct;22(5):735-6. doi: 10.4103/0970-9290.93475.
Single cell gel electrophoresis (SCGE) or "comet assay" is a rapid and very sensitive fluorescent microscopic method for detecting various forms of DNA damage at individual cell level.
The aim of the present study was to detect the extent of DNA damage in oral cancer, oral submucous fibrosis (OSMF) and leukoplakia in comparison to normal individual.
A total of 44 consecutive patients with oral cancer (n=26), leukoplakia (n=12) and OSMF (n=6) and 10 healthy normal volunteers with normal oral epithelia (controls) were recruited from Dr. R. Ahmed Dental College and Hospital and were assessed for the extent of DNA damage using SCGE following clinical diagnosis.
Peripheral blood was collected by venepuncture and comet assay was performed using SCGE. Mean tail length was compared between diagnostic groups and between different oral habit groups using t-tests and analysis of variance (ANOVA). Pearson's product moment correlation was used to examine the linear association between the extent of DNA damage and oral habit pack-years. Scheffe's pair-wise test was employed to adjust for multiple comparisons.
None of the controls were associated with any oral habits. Mean (±SD) tail lengths (in mm) for cancer (24.95 ± 5.09) and leukoplakia (12.96 ± 2.68) were significantly greater than in controls (8.54 ± 2.55, P<0.05). After adjustment, well-, moderately, and poorly differentiated carcinomas had significantly greater tail length than controls. Whereas the extent of DNA damage in cancer cases was significantly greater in leukoplakia than in compared to OSMF (11.03 ± 5.92), the DNA damage in latter was not different from controls. DNA damage for people with any oral habit (19.78 ± 7.77) was significantly greater than those with no habits (8.54 ± 2.55; P<0.0001).
DNA damage measured by SCGE is greater in leukoplakia and squamous cell carcinoma, but not in OSMF. Deleterious oral habits are also associated with greater DNA damage.
单细胞凝胶电泳（SCGE）或“彗星试验”是一种在单个细胞水平上检测各种形式DNA损伤的快速且非常灵敏的荧光显微镜方法。
本研究的目的是检测口腔癌、口腔黏膜下纤维化（OSMF）和白斑患者与正常个体相比的DNA损伤程度。
从R. Ahmed牙科医学院和医院招募了44例连续的口腔癌患者（n = 26）、白斑患者（n = 12）和OSMF患者（n = 6）以及10名口腔上皮正常的健康正常志愿者（对照组），在临床诊断后使用SCGE评估DNA损伤程度。
通过静脉穿刺采集外周血，并使用SCGE进行彗星试验。使用t检验和方差分析（ANOVA）比较诊断组之间以及不同口腔习惯组之间的平均尾长。使用Pearson积矩相关分析来检验DNA损伤程度与口腔习惯包年数之间的线性关联。采用Scheffe成对检验来调整多重比较。
对照组中无人有任何口腔习惯。癌症组（24.95±5.09）和白斑组（12.96±2.68）的平均（±标准差）尾长（以毫米为单位）显著大于对照组（8.54±2.55，P<0.05）。调整后，高分化、中分化和低分化癌的尾长显著大于对照组。虽然癌症病例中的DNA损伤程度在白斑组中显著大于OSMF组（11.03±5.92），但后者的DNA损伤与对照组无差异。有任何口腔习惯者的DNA损伤（19.78±7.77）显著大于无口腔习惯者（8.54±2.55；P<0.0001）。
通过SCGE测量的DNA损伤在白斑和鳞状细胞癌中更大，但在OSMF中则不然。有害的口腔习惯也与更大的DNA损伤有关。
