Yang Su-min, He Quan-ying, Miao Yi-de
People's Hospital, Peking University, Beijing 100044, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2003 Apr;26(4):203-5.
To investigate the relationship between the polymorphism of angiotensin converting enzyme (ACE) gene and ACE inhibitor (ACEI)-induced cough in Chinese elderly with essential hypertension.
ACE I/D polymorphism was performed on DNA samples from patients using the polymerase chain reaction (PCR) followed by agarose gel electrophoresis. Serum ACE levels were measured using a colorimetric assay.
The frequencies of ACE II genotype were 40% in patients with cough and 20% in those without cough, respectively (P < 0.05). The frequencies of I allele were 60% in patients with cough and 41% in those without cough (P < 0.01); The serum ACE level was highest in the DD genotype, followed by the ID genotype and the II genotype. The ACE level in patients with cough was significantly lower than that in patients without cough (P < 0.001). The sensitivity and specificity were 81% and 78% respectively when the serum ACE levels were used to predict ACEI-induced cough.
ACEI-induced cough was related to the serum ACE level and ACE gene polymorphism in Chinese elderly with essential hypertension.
探讨血管紧张素转换酶（ACE）基因多态性与老年中国原发性高血压患者中ACE抑制剂（ACEI）所致咳嗽之间的关系。
采用聚合酶链反应（PCR）及琼脂糖凝胶电泳对患者的DNA样本进行ACE I/D基因多态性检测。采用比色法测定血清ACE水平。
咳嗽患者中ACE II基因型频率分别为40%，无咳嗽患者中为20%（P < 0.05）。咳嗽患者中I等位基因频率为60%，无咳嗽患者中为41%（P < 0.01）；血清ACE水平在DD基因型中最高，其次是ID基因型和II基因型。咳嗽患者的ACE水平显著低于无咳嗽患者（P < 0.001）。当用血清ACE水平预测ACEI所致咳嗽时，敏感性和特异性分别为81%和78%。
老年中国原发性高血压患者中，ACEI所致咳嗽与血清ACE水平及ACE基因多态性有关。
