添加CCA的酶对tRNA的识别

tRNA recognition by CCA-adding enzyme.

作者信息

Tomari Yukihide, Suzuki Tsutomu, Ueda Takuya

机构信息

Department of Chemistry & Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):77-8. doi: 10.1093/nass/2.1.77.

DOI:10.1093/nass/2.1.77

PMID:12903113

Abstract

The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.

摘要

CCA添加酶[ATP（CTP）：tRNA核苷酸转移酶（EC. 2.7.7.25）]催化将保守的CCA序列添加到tRNA的3'末端。已知来自所有三个王国的CCA添加酶都能识别由T环和D环之间的三级相互作用形成的L形tRNA的肘部区域。我们在此通过克隆和表征重组蛋白，证明秀丽隐杆线虫线粒体CCA添加酶对线虫线粒体tRNA具有广泛的底物特异性，这些tRNA缺乏完整的T臂或D臂。