利用Cre/loxP（开启）系统开发依赖HIV-1的基因表达载体。

Development of HIV-1 dependent gene expression vector with the Cre/loxP (ON) system.

作者信息

Nagawa Takashi, Habu Yuichiro, Matsumoto Norihiko, Takeuchi Hiroaki, Kurosaki Naoko, Takaku Hiroshi

机构信息

Department of Industrial Chemistry, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):295-6. doi: 10.1093/nass/2.1.295.

DOI:10.1093/nass/2.1.295

PMID:12903221

Abstract

We used the HIV-1 5'LTR and the Cre/loxP system to develop an anti-gene expression system. The LTR promoter of HIV-1 has a specific activity that includes the intermediary region of gag, as shown in a previous report. We constructed the U5-region of HIV-1 as the target of the ribozyme expression vector (pCre/loxP-Rz), with the Cre/loxP system under the control of this LTR promoter. The function of this vector is to induce the HIV-1 dependent ribozyme-mediated inhibition in a dose-responsive manner. Furthermore, ribozyme mRNA expression was detected in the presence of pNL4-3 in HeLa-CD4+ cells. These studies are expected to yield novel applications of antiviral strategies for HIV-1.

摘要

我们利用HIV-1 5'长末端重复序列（LTR）和Cre/loxP系统开发了一种反基因表达系统。如先前报道所示，HIV-1的LTR启动子具有特定活性，其包括gag的中间区域。我们构建了HIV-1的U5区域作为核酶表达载体（pCre/loxP-Rz）的靶标，Cre/loxP系统受该LTR启动子的控制。该载体的功能是以剂量反应方式诱导HIV-1依赖性核酶介导的抑制作用。此外，在HeLa-CD4+细胞中存在pNL4-3的情况下检测到了核酶mRNA表达。这些研究有望为HIV-1抗病毒策略带来新的应用。