枯草芽孢杆菌组氨酸操纵子中组氨酸依赖性抗终止作用的分析。
Analysis of histidine-dependent antitermination in Bacillus subtilis hut operon.
作者信息
Oda M, Kobayashi N, Kurusu Y, Fujita M
机构信息
National Institute of Bioscience and Human Technology, AIST, MITI, Tsukuba Science City, Ibaraki 305-8566, Japan.
出版信息
Nucleic Acids Symp Ser. 2000(44):5-6. doi: 10.1093/nass/44.1.5.
We have previously shown that a positive regulator, HutP, of Bacillus subtilis hut operon is a RNA binding protein. Here, we report precise binding site of HutP in cis-regulatory region on hut mRNA and the role of HutP in histidine-dependent antitermination of hut expression. Ethylnitrosourea modification interference assay showed that four binding sites of HutP were found in the cis-regulatory sequences and were located at the stem and the internal loop of an antiterminator structure. In vitro transcription assay indicated that HutP suppressed transcription termination in the presence of histidine. These results suggest that HutP function as an antiterminator in response to the presence of histidine.
我们之前已经表明，枯草芽孢杆菌hut操纵子的一个正向调节因子HutP是一种RNA结合蛋白。在此，我们报告了HutP在hut mRNA顺式调节区域中的精确结合位点，以及HutP在组氨酸依赖性hut表达抗终止中的作用。乙基亚硝基脲修饰干扰试验表明，在顺式调节序列中发现了HutP的四个结合位点，它们位于抗终止子结构的茎和内环处。体外转录试验表明，在组氨酸存在的情况下，HutP抑制转录终止。这些结果表明，HutP作为一种抗终止子响应组氨酸的存在发挥作用。
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