Kubota Y, Kubota K, Tani S
Department of Chemistry, Faculty of Science, Yamaguchi University, Yamaguchi 753-8512, Japan.
Nucleic Acids Symp Ser. 2000(44):53-4. doi: 10.1093/nass/44.1.53.
DAPI analogs containing an imidazoline ring or a tetrahydropyrimidine ring have been synthesized to study DNA binding properties. Spectroscopic (absorption, CD, flow dichroism and fluorescence) and viscosity measurements indicate that DAPI analogs interact with DNA both by intercalation and by groove binding. The solution structures of complexes between DAPI analog and DNA oligomers have been characterized by proton NMR spectroscopy.
已合成含有咪唑啉环或四氢嘧啶环的DAPI类似物，以研究其与DNA的结合特性。光谱学（吸收光谱、圆二色光谱、流动二色光谱和荧光光谱）和粘度测量表明，DAPI类似物通过嵌入和沟槽结合两种方式与DNA相互作用。已通过质子核磁共振光谱对DAPI类似物与DNA寡聚物之间复合物的溶液结构进行了表征。
