Masuda K, Nakata T, Tamagake K
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
Nucleic Acids Symp Ser. 2000(44):63-4. doi: 10.1093/nass/44.1.63.
We have been investigated the possibility of B-Z transition in ZnTMPyP-DNA interaction based on the observation of spectroscopic data. In this study, we found drastic change in the AFM image of supercoiled plasmid DNA when it was interacted with TMPyPs indicating that the considerable amount of unwinding of double helix or B-Z transition is induced by the drug-DNA interaction. Such phenomena were not observed for other cationic drugs examined.
基于光谱数据的观察，我们一直在研究锌四（N-甲基吡啶）卟啉（ZnTMPyP）与DNA相互作用中B-Z转变的可能性。在本研究中，我们发现当超螺旋质粒DNA与四（N-甲基吡啶）卟啉（TMPyPs）相互作用时，其原子力显微镜（AFM）图像发生了剧烈变化，这表明药物与DNA的相互作用诱导了相当数量的双螺旋解旋或B-Z转变。对于所检测的其他阳离子药物，未观察到此类现象。
