Zama M
National Institute of Radiological Sciences, 9-1 Anagawa-4-chome, Inage-ku, Chiba 263-8555, Japan.
Nucleic Acids Symp Ser. 2000(44):91-2. doi: 10.1093/nass/44.1.91.
An analysis of the published data of the electrophoresis of the products of silk fibroin exhibiting discontinuities or pauses in the translational process showed that about 60 times of pauses occur during the translation of the mRNA from silk fibroin. In addition, we estimated that silk fibroin gene as a whole (15 kb) is composed of about 60 alternate, crystalline-noncrystalline, arrays of nucleotide sequence elements each connected by the boundary sequence, on the basis of the nucleotide sequence of the partial cDNA clone of the heavy chain gene of Bombyx mori available in the literature. The coincidence of the total number of pauses with that of the alternate arrays suggests that one translational pausing site exists in each of the unit (alternate array + boundary) nucleotide sequence in the silk fibroin mRNA template.
对丝素蛋白产物电泳的已发表数据进行分析，这些产物在翻译过程中表现出不连续性或停顿，结果表明，在丝素蛋白mRNA的翻译过程中大约会出现60次停顿。此外，根据文献中家蚕重链基因部分cDNA克隆的核苷酸序列，我们估计丝素蛋白基因整体（15kb）由大约60个交替的、结晶-非结晶的核苷酸序列元件阵列组成，每个阵列由边界序列连接。停顿总数与交替阵列总数的巧合表明，丝素蛋白mRNA模板中的每个单位（交替阵列+边界）核苷酸序列中存在一个翻译停顿位点。
