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Genome structure of Ri plasmid (3). Sequencing analysis of the vir region of pRi1724 in Japanese Agrobacterium rhizogenes.

作者信息

Satuti N, Moriguchi K, Sato M, Kataoka M, Maeda Y, Tanaka N, Yoshida K

机构信息

Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8256, Japan.

出版信息

Nucleic Acids Symp Ser. 2000(44):95-6. doi: 10.1093/nass/44.1.95.

DOI:10.1093/nass/44.1.95
PMID:12903285
Abstract

The entire genome of the pRi1724 (217.6-kb) in the mikimopine type Agrobacterium rhizogenes strain MAFF03-01724 has been completely sequenced. The vir region covering 30.2-kb has found to be composed of 21 genes resembling virH1, virA, virB1-11, virG, virC1-2, and virD1-5. The structural organization of the pRi1724 vir operons in this study is exactly the same as that of the previously reported vir operons of other Ri or Ti plasmids, although the size of some ORFs showed little variations among the plasmids. We also found virE3 gene in the pRi1724 (1), but different from Ti plasmids, virE1 and virE2 that are also important for the virulence do not exist in the vir region of pRi1724.

摘要

相似文献

1
Genome structure of Ri plasmid (3). Sequencing analysis of the vir region of pRi1724 in Japanese Agrobacterium rhizogenes.
Nucleic Acids Symp Ser. 2000(44):95-6. doi: 10.1093/nass/44.1.95.
2
Genome structure of Ri plasmid (2). Sequencing analysis of T-DNA and its flanking regions of pRi1724 in Japanese Agrobacterium rhizogenes.发根质粒(Ri)的基因组结构(2)。发根土壤杆菌pRi1724的T-DNA及其侧翼区域的测序分析。
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The complete nucleotide sequence of a plant root-inducing (Ri) plasmid indicates its chimeric structure and evolutionary relationship between tumor-inducing (Ti) and symbiotic (Sym) plasmids in Rhizobiaceae.一种植物致根(Ri)质粒的完整核苷酸序列表明了其嵌合结构以及根瘤菌科中致瘤(Ti)质粒和共生(Sym)质粒之间的进化关系。
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Virulence genes promote conjugative transfer of the Ti plasmid between Agrobacterium strains.毒力基因促进根癌土壤杆菌菌株之间Ti质粒的接合转移。
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Efficient vir gene induction in Agrobacterium tumefaciens requires virA, virG, and vir box from the same Ti plasmid.根癌农杆菌中高效的vir基因诱导需要来自同一Ti质粒的virA、virG和vir盒。
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The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes.调控蛋白VirG特异性结合参与根癌土壤杆菌毒力基因转录激活的顺式作用调控序列。
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A comparison of virulence determinants in an octopine Ti plasmid, a nopaline Ti plasmid, and an Ri plasmid by complementation analysis of Agrobacterium tumefaciens mutants.通过根癌土壤杆菌突变体的互补分析比较章鱼碱型Ti质粒、胭脂碱型Ti质粒和Ri质粒中的毒力决定因素。
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