Hohsaka T, Sisido M
Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Nucleic Acids Symp Ser. 2000(44):99-100. doi: 10.1093/nass/44.1.99.
A novel strategy for the incorporation of nonnatural amino acids into proteins was developed by using five-base codon-anticodon pairs. The streptavidin mRNA containing five-base codon CGGUA and the chemically aminoacylated tRNA with five-base anticodon UACCG were prepared, and added into E. coli in vitro translation system. As a result, the nonnatural amino acid was successfully incorporated into desired position of the protein. Other five-base codons CGGN1N2, where N1 and N2 indicate one of four nucleotides, were also available for the incorporation of the nonnatural amino acid.
通过使用五碱基密码子-反密码子对，开发了一种将非天然氨基酸掺入蛋白质的新策略。制备了含有五碱基密码子CGGUA的链霉亲和素mRNA和带有五碱基反密码子UACCG的化学氨酰化tRNA，并将其添加到大肠杆菌体外翻译系统中。结果，非天然氨基酸成功掺入到蛋白质的所需位置。其他五碱基密码子CGGN1N2（其中N1和N2表示四种核苷酸之一）也可用于掺入非天然氨基酸。
