基于石英晶体微天平的RNA适配体识别富含精氨酸基序（ARM）模型肽的体外筛选与分析
In vitro selection and analysis of RNA aptamer recognize arginine-rich motif (ARM) model peptide on a QCM.
作者信息
Fukusho S, Furusawa H, Okahata Y
机构信息
Department of Biomolecular Engineering, Tokyo Institute of Technology, Nagatsuda, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.
出版信息
Nucleic Acids Symp Ser. 2000(44):187-8. doi: 10.1093/nass/44.1.187.
PMID:12903331
Abstract
To study RNA-peptide interactions, we performed an in vitro selection of RNA on a 27 MHz quartz-crystal microbalance (QCM) on which a simple R5 helix peptide was immobilized as a model of N peptide from bacteriophade lambda. The consensus sequences including a GNRA tetraloop were obtained from a random RNA pool after the 7th cycle selection.
摘要
为了研究RNA与肽的相互作用，我们在一台27兆赫兹的石英晶体微天平（QCM）上对RNA进行了体外筛选，在该天平上固定了一个简单的R5螺旋肽作为来自噬菌体λ的N肽模型。经过第7轮筛选后，从随机RNA库中获得了包含GNRA四环的共有序列。
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