马铃薯块茎（品种为埃尔卡纳）中含量最丰富的蛋白酶抑制剂是一种来自库尼茨家族的丝氨酸蛋白酶抑制剂。
The most abundant protease inhibitor in potato tuber (cv. Elkana) is a serine protease inhibitor from the Kunitz family.
作者信息
Pouvreau Laurice, Gruppen Harry, Van Koningsveld Gerrit A, Van Den Broek Lambertus A M, Voragen Alphons G J
机构信息
Centre for Protein Technology TNO-WU, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
出版信息
J Agric Food Chem. 2003 Aug 13;51(17):5001-5. doi: 10.1021/jf0212486.
The gene of the most abundant protease inhibitor in potato cv. Elkana was isolated and sequenced. The deduced amino acid sequence of this gene showed 98% identity with potato serine protease inhibitor (PSPI), a member of the Kunitz family. Therefore, the most abundant protease inhibitor was considered to be one of the isoforms of PSPI. The PSPI group represents approximately 22% of the total amount of proteins in potato cv. Elkana and is composed of seven different isoforms that slightly differ in isoelectric point. Antibodies were raised against the two most abundant isoforms of PSPI. The binding of these antibodies to PSPI isoforms and protease inhibitors from different groups of protease inhibitor in potato showed that approximately 70% of the protease inhibitors present in potato juice belong to the Kunitz family.
对马铃薯品种埃尔卡纳（cv. Elkana）中最丰富的蛋白酶抑制剂基因进行了分离和测序。该基因推导的氨基酸序列与马铃薯丝氨酸蛋白酶抑制剂（PSPI，库尼茨家族成员之一）具有98%的同一性。因此，这种最丰富的蛋白酶抑制剂被认为是PSPI的同工型之一。PSPI组约占马铃薯品种埃尔卡纳总蛋白量的22%，由七种等电点略有不同的不同同工型组成。制备了针对PSPI两种最丰富同工型的抗体。这些抗体与马铃薯中不同蛋白酶抑制剂组的PSPI同工型和蛋白酶抑制剂的结合表明，马铃薯汁中约70%的蛋白酶抑制剂属于库尼茨家族。
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