Speranskaia A S, Dorokhin A V, Novikova S I, Shevelev A B, Valueva T A
Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071 Russia.
Genetika. 2003 Nov;39(11):1490-7.
Seven structurally similar clones from potato (Solanum tuberosum L.), cv. Istrinskii genomic DNA were isolated by cloning of the PCR products. It was suggested that five of these clones were the amplified copies of the same gene. Based on comparative and structural analysis of these clones, initial nucleotide structure of the gene was reconstructed. It appeared to be highly homologous (98%) to the already published sequences encoding the proteins belonging to the soybean Kunitz trypsin inhibitor family (SKTI). Comparison of the results with the previously published data on the SKTI-type proteinase inhibitors from potato of cv. Istrinskii suggests that the gene examined encodes both chains of the earlier described PSPI-21-6.3 protein [9].
通过克隆聚合酶链式反应（PCR）产物，从马铃薯（Solanum tuberosum L.）品种Istrinskii的基因组DNA中分离出七个结构相似的克隆。据推测，其中五个克隆是同一基因的扩增拷贝。基于对这些克隆的比较和结构分析，重建了该基因的初始核苷酸结构。结果表明，它与已发表的编码大豆Kunitz胰蛋白酶抑制剂家族（SKTI）蛋白质的序列高度同源（98%）。将该结果与之前发表的关于马铃薯品种Istrinskii的SKTI型蛋白酶抑制剂的数据进行比较，表明所检测的基因编码了先前描述的PSPI-21-6.3蛋白的两条链[9]。
