Rathjen Joy, Washington Jennifer M, Bettess Michael D, Rathjen Peter D
School of Molecular and Biomedical Sciences, and Australian Research Council Special Research Centre for Molecular Genetics of Development, University of Adelaide, Adelaide, South Australia 5005, Australia.
Biol Reprod. 2003 Dec;69(6):1863-71. doi: 10.1095/biolreprod.103.017384. Epub 2003 Aug 6.
Pluripotent cell development in the mammalian embryo results in the sequential formation of several developmentally distinct populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2 cells (MEDII) have been implicated in the formation and maintenance of primitive ectoderm from inner cell mass cells both in vitro and in vivo. Here we demonstrate that MEDII, but not LIF, is able to support the maintenance and proliferation in culture of pluripotent cells derived from primitive ectoderm formed in vitro or during embryonic development. This distinguishes primitive ectoderm and inner cell mass (ICM) on the basis of cytokine responsiveness and validates the biological activity proposed for factors within MEDII in primitive ectoderm establishment and maintenance. Further, it potentially provides an alternative technology for the isolation of pluripotent cells from the mammalian embryo.
哺乳动物胚胎中的多能细胞发育会导致依次形成几个发育上不同的群体，即内细胞团、原始外胚层和原始生殖谱系。由HepG2细胞条件培养基（MEDII）中的因子已被证明在体外和体内都参与了从内细胞团细胞形成和维持原始外胚层的过程。在这里，我们证明MEDII，而不是LIF，能够支持源自体外形成或胚胎发育过程中形成的原始外胚层的多能细胞在培养中的维持和增殖。这在细胞因子反应性的基础上区分了原始外胚层和内细胞团（ICM），并验证了MEDII中因子在原始外胚层建立和维持中所提出的生物活性。此外，它可能为从哺乳动物胚胎中分离多能细胞提供一种替代技术。
