Kaetzke Annette, Körner Henrike, Kneist Susanne, Eschrich Klaus
Institute of Biochemistry, Medical Faculty, University of Leipzig, D-04103 Leipzig, Germany.
J Clin Microbiol. 2003 Aug;41(8):3729-31. doi: 10.1128/JCM.41.8.3729-3731.2003.
The accurate classification of oral Actinomyces isolates as one species is difficult. Out of 18 Actinomyces isolates forming red colonies on brain heart blood agar, 12 could be straightforwardly assigned as Actinomyces odontolyticus by biochemical, morphological, and chemotaxonomic characteristics. For the remaining six isolates, the results of the different identification methods were inconsistent. By sequencing a 16S ribosomal DNA fragment by a rapid mass spectrometric method, all isolates could be identified unambiguously as A. odontolyticus. This result proves the importance of red colony pigmentation on brain heart blood agar together with the characteristic cell morphology for unequivocal assignment of oral Actinomyces isolates to the species A. odontolyticus.
将口腔放线菌分离株准确分类为单一物种很困难。在18株于脑心血琼脂上形成红色菌落的放线菌分离株中，根据生化、形态学和化学分类学特征，有12株可直接归类为溶齿放线菌。对于其余6株分离株，不同鉴定方法的结果并不一致。通过快速质谱法对16S核糖体DNA片段进行测序，所有分离株均可明确鉴定为溶齿放线菌。这一结果证明了脑心血琼脂上红色菌落色素沉着以及特征性细胞形态对于将口腔放线菌分离株明确归类为溶齿放线菌的重要性。
