用于检测嗜吞噬细胞无形体的定量实时聚合酶链反应检测法。
Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis.
作者信息
Loftis Amanda D, Massung Robert F, Levin Michael L
机构信息
Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 2003 Aug;41(8):3870-2. doi: 10.1128/JCM.41.8.3870-3872.2003.
Abstract
A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis. The assay is species specific and provides quantitative results in the range 10 to 10(10) gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis.
摘要
开发了一种用于检测恰菲埃立克体的实时聚合酶链反应（PCR）检测方法。该检测方法具有种属特异性，可提供10至10（10）个基因拷贝范围内的定量结果。该检测方法不受蜱、人或小鼠DNA的存在影响，并且与高通量样本兼容。将该检测方法与先前描述的恰菲埃立克体检测方法进行了比较。
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J Immunol. 2002 Aug 1;169(3):1419-25. doi: 10.4049/jimmunol.169.3.1419.
3
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4
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