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用于检测嗜吞噬细胞无形体的定量实时聚合酶链反应检测法。

Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis.

作者信息

Loftis Amanda D, Massung Robert F, Levin Michael L

机构信息

Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

出版信息

J Clin Microbiol. 2003 Aug;41(8):3870-2. doi: 10.1128/JCM.41.8.3870-3872.2003.

DOI:10.1128/JCM.41.8.3870-3872.2003
PMID:12904406
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC179802/
Abstract

A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis. The assay is species specific and provides quantitative results in the range 10 to 10(10) gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis.

摘要

开发了一种用于检测恰菲埃立克体的实时聚合酶链反应（PCR）检测方法。该检测方法具有种属特异性，可提供10至10（10）个基因拷贝范围内的定量结果。该检测方法不受蜱、人或小鼠DNA的存在影响，并且与高通量样本兼容。将该检测方法与先前描述的恰菲埃立克体检测方法进行了比较。

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本文引用的文献

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DNA Cell Biol. 2002 Aug;21(8):587-96. doi: 10.1089/104454902320308960.
2
Antibodies highly effective in SCID mice during infection by the intracellular bacterium Ehrlichia chaffeensis are of picomolar affinity and exhibit preferential epitope and isotype utilization.在感染细胞内细菌恰菲埃立克体期间，对重症联合免疫缺陷（SCID）小鼠高度有效的抗体具有皮摩尔亲和力，并表现出优先的表位和同种型利用。
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Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila.立克次氏体目里克次体科和无形体科属的重新分类：将埃立克体属的一些物种与无形体属合并、考德里体属与埃立克体属合并以及埃立克体属与新立克次体属合并，六个新物种组合的描述以及将马埃立克体和“人粒细胞埃立克体病病原体”指定为嗜吞噬细胞埃立克体的主观同义词。
Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):2145-2165. doi: 10.1099/00207713-51-6-2145.
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Amblyomma americanum: a potential vector of human ehrlichiosis.美洲钝眼蜱：人类埃立克体病的潜在传播媒介。
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