Cloning PCR products with T-vectors.
作者信息
Nichols Wade A
机构信息
Department of Biological Sciences, Illinois State University, Normal, USA.
出版信息
Methods Mol Biol. 2003;235:141-52. doi: 10.1385/1-59259-409-3:141.
PMID:12904657
Abstract
摘要
相似文献
1
Cloning PCR products with T-vectors.使用T载体克隆PCR产物。
Methods Mol Biol. 2003;235:141-52. doi: 10.1385/1-59259-409-3:141.
2
Construction of an XcmI-generated T vector bearing green fluorescent protein marker for direct cloning of PCR products.构建用于直接克隆PCR产物的携带绿色荧光蛋白标记的XcmI酶切产生的T载体。
Anal Biochem. 2007 Jan 1;360(1):144-5. doi: 10.1016/j.ab.2006.10.031. Epub 2006 Nov 7.
3
[A novel T-cloning vector system].[一种新型的T克隆载体系统]
Mikrobiyol Bul. 2004 Jul;38(3):239-43.
4
Adenosine added on the primer 5' end improved TA cloning efficiency of polymerase chain reaction products.
Anal Biochem. 2007 Apr 1;363(1):163-5. doi: 10.1016/j.ab.2007.01.014. Epub 2007 Jan 17.
5
PCR cloning using T-vectors.使用T载体进行PCR克隆。
Methods Mol Biol. 1994;31:19-33. doi: 10.1385/0-89603-258-2:19.
6
Restriction selection cloning: a simple general method for the selection of recombinant DNA.限制性选择克隆：一种用于筛选重组DNA的简单通用方法。
Cell Mol Biol Res. 1993;39(2):177-82.
7
Cloning in plasmid vectors.
Methods Mol Biol. 2003;235:121-35. doi: 10.1385/1-59259-409-3:121.
8
Construction of a restriction-endonuclease-Eam1105I-generated T-vector for high-throughput cloning and expression.用于高通量克隆和表达的由限制性内切酶Eam1105I构建的T载体
Biotechnol Appl Biochem. 2007 Sep;48(Pt 1):29-33. doi: 10.1042/BA20070033.
9
Cloning PCR products using T-vectors.
Methods Mol Biol. 1996;58:313-24. doi: 10.1385/0-89603-402-X:313.
10
Direct cloning of unmodified PCR products by exploiting an engineered restriction site.通过利用工程化限制酶切位点直接克隆未修饰的PCR产物。
Gene. 1994 May 27;143(1):151-2. doi: 10.1016/0378-1119(94)90623-8.
引用本文的文献
1
A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products.一种新型高效 TA 克隆和 PCR 产物平末端克隆载体系列。
Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.1038/s41598-019-42868-6.
Zotero 插件