[双能X线吸收法体外测量大鼠骨密度的精度]

[Precision of rat BMD measurements by dual-energy X-ray absorptiometry in vitro].

作者信息

Xing Xiao-ping, Meng Xun-wu, Cheng Zhi-qiang, Zhan Zhi-wei, Chui Zhi-hui, Xia Wei-bo, Zhou Xue-ying

机构信息

Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Jun;24(3):302-5.

PMID:12905640

Abstract

OBJECTIVE

To evaluate the precision of rat bone mineral density (BMD) measurements by Norland Excellplus dual-energy X-ray absorptiometry (DXA) and to investigate the BMD changes in ovariectomized (Ovx) rats in vitro.

METHODS

(1) The coefficients of variation (CV) for BMD measurements at various skeletal regions were repeatedly determined by DXA in 10 Wistar rats in vitro. (2) BMD in lumbar vertebra (L5) and both sides of femurs was measured in total 90 rats. And (3) changes in BMD between Ovx and sham rats were compared.

RESULTS

(1) The short-term CVs of BMD measurements in different regions by DXA were as follows, 1.58% for lumbar vertebra (L5), 0.90% for left femur, and 0.86% for right femur, respectively. The long-term CVs were 2.22% for lumbar vertebra (L5), 1.09% for left femur, and 1.20% for right femur. (2) The BMD values in 90 Wistar rats were (127.5 +/- 12.3) in lumbar vertebra (L5), (82.6 +/- 11.3) in corpus vertebra (L5'), (150.7 +/- 10.6) in left femur and (149.9 +/- 10.6) mg/cm2 in right femur, respectively. The correlation coefficient of BMD measurements between left and right femurs was 0.792 (P < 0.001). (3) In Ovx group, the BMD values of corpus vertebra (L5') and distal femurs were significantly decreased, that was 10.0%-17.5% lower in comparison with sham group (P < 0.001).

CONCLUSIONS

Measurement of rat BMD in vitro by Norland Excellplus DXA is a useful method, and it can reflect the changes in rat bone masses with good precision.

摘要

目的

评估Norland Excellplus双能X线吸收法（DXA）测量大鼠骨密度（BMD）的精度，并在体外研究去卵巢（Ovx）大鼠的骨密度变化。

方法

（1）在体外，通过DXA对10只Wistar大鼠不同骨骼区域的骨密度测量变异系数（CV）进行重复测定。（2）对总共90只大鼠测量腰椎（L5）和双侧股骨的骨密度。并且（3）比较Ovx大鼠和假手术大鼠之间的骨密度变化。

结果

（1）DXA测量不同区域骨密度的短期变异系数如下，腰椎（L5）为1.58%，左侧股骨为0.90%，右侧股骨为0.86%。长期变异系数分别为，腰椎（L5）为2.22%，左侧股骨为1.09%，右侧股骨为1.20%。（2）90只Wistar大鼠的骨密度值分别为，腰椎（L5）为（127.5±12.3），椎体（L5'）为（82.6±11.3），左侧股骨为（150.7±10.6），右侧股骨为（149.9±10.6）mg/cm²。左右股骨骨密度测量的相关系数为0.792（P<0.001）。（3）在Ovx组，椎体（L5'）和股骨远端的骨密度值显著降低，与假手术组相比降低了10.0%-17.5%（P<0.001）。