Sun Qiu-ning, Fang Kai
Department of Dermatology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Aug;24(4):385-8.
To establish a model of Candida biofilm and to explore its characteristics, ultrastructure, influences by saliva and serum, and sensitivity to antifungal agents.
Evaluations of the in vitro growth kinetics, influences by saliva and serum, and sensitivity to antifungal agents of Candida biofilm were performed with the abated tetrazolium salt XTT method on a 96-well microtire petri dish. The ultrastructure of Candida biofilm was observed under Confocal Laser Scanning Microscope (CLSM).
The bioactivity of Candida biofilm increased with culturing time and serum could obviously increase the action of biofilm. The Candida biofilm was significantly resistant to routine antifungal agents.
The Candida cells adhered in biofilms are significantly different in morphology from those in suspension and are resistant to routine antifungal agents such as Amphotericine B, Fluconazole and Itraconazole.
建立念珠菌生物膜模型，探讨其特性、超微结构、受唾液和血清的影响以及对抗真菌药物的敏感性。
采用减弱的四氮唑盐XTT法在96孔微量培养板上对念珠菌生物膜的体外生长动力学、受唾液和血清的影响以及对抗真菌药物的敏感性进行评估。在共聚焦激光扫描显微镜（CLSM）下观察念珠菌生物膜的超微结构。
念珠菌生物膜的生物活性随培养时间增加，血清可明显增强生物膜的活性。念珠菌生物膜对常规抗真菌药物具有显著抗性。
生物膜中黏附的念珠菌细胞在形态上与悬浮状态的细胞有显著差异，且对两性霉素B、氟康唑和伊曲康唑等常规抗真菌药物具有抗性。
