Hu Yao-fei, Ren Zu-yuan, Li Yun-feng, Sun Hong-xia, Chang Yong-sheng, Su Chang-bao, Wang Ren-zhi, Zuo Jin, Fang Fu-de
Department of Neurosurgery, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Dec;24(6):611-5.
To observe the characteristics and difference of gene expression in the pituitary adenomas and para-tumor normal pituitary tissues.
Using serial analysis of gene expression (SAGE), two SAGE libraries were generated. Forty clones from each SAGE library were sequenced, and the results were analyzed by SAGE2000 software and compared with the SAGE map at NCBI.
A total of 655 gene tags, representing 43 genes, were extracted from the 40 sequence files of the para-tumor normal pituitary tissues and 737 gene tags, representing 53 genes, were extracted from the 40 sequence files of the pituitary adenomas. Of these tags, 13 were not reported before. The genes related to pituitary hormone secretion and energy metabolism were highly expressed in the two kinds of tissues. Some growth factors and cytokines were also expressed, including those involved in the immunological system. But there were also much difference of gene expression in the two tissues. Thirty-one and five tags were only detected in para-tumor normal pituitary tissues and pituitary adenomas, respectively.
Genes involved in hormones secretion and energy metabolism were highly expressed in the pituitary adenomas and para-tumor normal pituitary tissues. Many growth factors and cytokines were also expressed in pituitary. There was also much difference of gene expression in the two kinds of tissues. SAGE can be used not only in understanding the quantity information of gene expression, but also in finding new genes.
观察垂体腺瘤及瘤旁正常垂体组织中基因表达的特点及差异。
采用基因表达序列分析（SAGE）技术构建两个SAGE文库。从每个SAGE文库中选取40个克隆进行测序，结果用SAGE2000软件分析，并与NCBI的SAGE图谱进行比较。
从瘤旁正常垂体组织的40个序列文件中提取出代表43个基因的655个基因标签，从垂体腺瘤的40个序列文件中提取出代表53个基因的737个基因标签。其中有13个标签此前未见报道。与垂体激素分泌和能量代谢相关的基因在两种组织中均高表达。还表达了一些生长因子和细胞因子，包括参与免疫系统的因子。但两种组织的基因表达也存在很大差异。分别在瘤旁正常垂体组织和垂体腺瘤中仅检测到31个和5个标签。
参与激素分泌和能量代谢的基因在垂体腺瘤及瘤旁正常垂体组织中高表达。垂体中也表达了许多生长因子和细胞因子。两种组织的基因表达也存在很大差异。SAGE不仅可用于了解基因表达的数量信息，还可用于发现新基因。
