Zhang Jian, Du Ye-wei, Sun Ren-yu, Wang Shi-wen
Department of Pathophysiology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Jun;25(3):320-3.
To investigate whether acute lung injury (ALI) and changes of myocardial ATP enzymes were induced by intravenous or intraventricle of left heart injection of lipopolysaccharide (LPS) in aging rats.
40 male Wistar rats were used for reproducing aging animal model. Aging rats were randomly divided into aging control group (n = 8), ALI group (LPS, 5 mg/kg body weight intravenous injection, n = 16), and LPS group (same dosage LPS, intraventricle of left heart injection, n = 16). The samples (blood, lung and heart) were collected at 2, 6 hours after LPS or saline administration.
Compared with aging control, protein content in bronchial alveolar lavage fluid (BALF), ratio of lung wet/dry weight and the LA, NO2-/NO3- and MDA contents in blood were increased markedly (P < 0.01) at 2, 6 hours in ALI group. The GSH-Px, Na(+)-K(+)-ATPase activities in lung tissue were decreased significantly (P < 0.01), but NO2-/NO3- content in lung tissue was increased obviously (P < 0.01) at 2 hours in ALI group. These changes were maintained until at 6 hours after LPS administration. The above parameters were no obviously changes in myocardium at 2 hours after LPS administration in ALI group. But at 6 hours, MDA content was increased obviously (P < 0.01); Na(+)-K(+)-ATPase, Ca(2+)-Mg(2+)-ATPase and GSH-Px activities in myocardium were decreased markedly (P < 0.01). While in LPS group, only NO2-/NO3- contents were increased (P < 0.05) in blood and lung tissue as well as Na(+)-K(+)-ATPase activity in lung tissue were decreased (P < 0.05), another parameters had no obvious changes.
ALI was obviously formed by intravenous injection LPS after 2, 6 hours in aging rats. Myocardial enzyme etc decreased only at 6 hours in ALI group. But above parameters were no obviously changes in LPS group. It was suggested that there was probable myocardial damage in rats of ALI group, and it was mainly induced by ALI.
研究静脉注射或左心室内注射脂多糖（LPS）是否可诱导老年大鼠发生急性肺损伤（ALI）及心肌ATP酶的变化。
选用40只雄性Wistar大鼠建立老年动物模型。将老年大鼠随机分为老年对照组（n = 8）、ALI组（LPS，5 mg/kg体重静脉注射，n = 16）和LPS组（相同剂量LPS左心室内注射，n = 16）。在给予LPS或生理盐水后2、6小时采集样本（血液、肺和心脏）。
与老年对照组相比，ALI组在给予LPS后2、6小时支气管肺泡灌洗液（BALF）中的蛋白含量、肺湿/干重比值以及血液中LA、NO2-/NO3-和MDA含量均显著升高（P < 0.01）。ALI组肺组织中GSH-Px、Na(+)-K(+)-ATP酶活性在2小时时显著降低（P < 0.01），但肺组织中NO2-/NO3-含量在2小时时明显升高（P < 0.01）。这些变化一直持续到给予LPS后6小时。ALI组在给予LPS后2小时心肌上述参数无明显变化。但在6小时时，MDA含量明显升高（P < 0.01）；心肌中Na(+)-K(+)-ATP酶、Ca(2+)-Mg(2+)-ATP酶和GSH-Px活性显著降低（P < 0.01）。而在LPS组，仅血液和肺组织中NO2-/NO3-含量升高（P < 0.05），肺组织中Na(+)-K(+)-ATP酶活性降低（P < 0.05），其他参数无明显变化。
老年大鼠静脉注射LPS后2、6小时明显形成ALI。ALI组心肌酶等仅在6小时时降低。但LPS组上述参数无明显变化。提示ALI组大鼠可能存在心肌损伤，且主要由ALI诱导。
