Zhang Jian, Zeng Wu-wei, Chen Bao-sheng, Wu Gang, Zhang Wen-cheng, Zhang Ke-man
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Apr;24(2):149-55.
To acquire cDNA sequence of lecithin-cholesterol acyltransferase (LCAT) from tree shrew and analyze the sequence structure.
The first strand cDNA was acquired by reverse transcription using mRNA from tree shrew liver as template. By the method of SMART RACE PCR, tree shrew LCAT cDNA was acquired and deduced its amino acids sequence. The sequence and structure of tree shrew LCAT cDNA and amino acid were analyzed and predicted by the molecular software.
Tree shrew LCAT cDNA is composed of 1,340 bp, including 2 bp 5' untranslated region (5' UTR), 1,320 bp open reading frame (ORF) which encodes protein precursor of 440 amino acids (24 amino acids signal peptide and 416 amino acids mature peptide), and 18 bp 3' untranslated region (3'UTR). The stop codon is TAA and there is a poly (A) signal sequence AATAAA and a 25 bp poly (A) tail. Tree shrew LCAT cDNA sequence has been accepted by GenBank as a new gene, accession number AF272861 and its homology with human and baboon was 90% and 89%, respectively.
The sequence of LCAT cDNA in tree shrew has high identity with that of human and other experimental animal species.
获取树鼩卵磷脂胆固醇酰基转移酶（LCAT）的cDNA序列并分析其序列结构。
以树鼩肝脏mRNA为模板，通过反转录获得第一链cDNA。采用SMART RACE PCR方法获取树鼩LCAT cDNA并推导其氨基酸序列。利用分子软件对树鼩LCAT cDNA及氨基酸的序列和结构进行分析和预测。
树鼩LCAT cDNA由1340 bp组成，包括2 bp的5'非翻译区（5'UTR）、1320 bp的开放阅读框（ORF），其编码440个氨基酸的蛋白质前体（24个氨基酸的信号肽和416个氨基酸的成熟肽）以及18 bp的3'非翻译区（3'UTR）。终止密码子为TAA，存在多聚（A）信号序列AATAAA和25 bp的多聚（A）尾。树鼩LCAT cDNA序列已被GenBank收录为新基因，登录号为AF272861，其与人及狒狒的同源性分别为90%和89%。
树鼩LCAT cDNA序列与人及其他实验动物物种的序列具有高度同源性。
