[恶性疟原虫含PDZ结构域蛋白新全长开放阅读框基因的鉴定与克隆]
[Identification and cloning of a novel full ORF gene of P. falciparum PDZ domain containing protein].
作者信息
Li H L, Wang H
机构信息
Department of Etiology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001 Oct;23(5):458-61.
OBJECTIVE
To identify and clone a novel full ORF gene of P. falciparum PDZ domain containing protein (PfPCP) and study its expression at the erythrocytic stage.
METHODS
The primers were designed according to the sequences from P. falciparum genomic database. The full ORF gene of PfPCP was amplified from mRNA of the erythrocytic stage of P. falciparum Hainan strain by RT-PCR. Bio-informatics software were used to analyze PfPCP; PfPCP expression at the erythrocytic stage was studied with Western Blotting.
RESULTS
The full ORF gene of PfPCP was 2,076 bp and encoded 691 amino acids. PfPCP contained the typical PDZ domain. Western blotting showed that PfPCP was expressed at the schizont sub-stage of P. falciparum erythrocytic stage.
CONCLUSIONS
The novel full ORF gene of PfPCP is cloned. As a PDZ domain containing protein, PfPCP is expressed at the schizont sub-stage of P. falciparum.
目的
鉴定并克隆恶性疟原虫含PDZ结构域蛋白（PfPCP）的一个新的全长开放阅读框基因，并研究其在红细胞内期的表达。
方法
根据恶性疟原虫基因组数据库中的序列设计引物。通过RT-PCR从恶性疟原虫海南株红细胞内期的mRNA中扩增PfPCP的全长开放阅读框基因。使用生物信息学软件分析PfPCP；采用蛋白质免疫印迹法研究PfPCP在红细胞内期的表达。
结果
PfPCP的全长开放阅读框基因为2076 bp，编码691个氨基酸。PfPCP含有典型的PDZ结构域。蛋白质免疫印迹显示PfPCP在恶性疟原虫红细胞内期的裂殖体亚期表达。
结论
克隆了PfPCP的新全长开放阅读框基因。作为一种含PDZ结构域的蛋白，PfPCP在恶性疟原虫的裂殖体亚期表达。
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