[一种用于正确记录听神经和下丘单位放电的方法]
[An approach for proper recording of the unit discharge in auditory nerve and inferior colliculus].
作者信息
Pan T, Cao K L, Wang Z Z
机构信息
ENT Department, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001 Oct;23(5):481-4.
OBJECTIVE
To explore a proper recording of the unit discharge in auditory nerve and inferior colliculus for the observation of characteristics of response potential.
METHODS
Single unit discharge in auditory nerve and inferior colliculus of healthy adult cat to acoustic stimulation and intracochlear electrical stimulation were recorded. With glass microelectrode.
RESULTS
(1) the spontaneous discharge in auditory nerve primary fibers were identified. (2) The waveform of the spontaneous discharge in auditory nerve primary fibers was biphasic potential with positive pulse width 0.5 ms. (3) The discharge rate of auditory nerve ranged from several PPS to 100PPS. (4) The response in inferior colliculus to high frequency pure tone stimulation lacked phase locking. (5) The response in inferior colliculus to low frequency electrical stimulation in cochlea showed phase locking.
CONCLUSIONS
It was successful to record the spontaneous discharges and auditory evoked responses in auditory nerve as well as the pure tone evoked response and electrically evoked response in inferior colliculus. However, the existing technical problem in recording the electrically evoked response in auditory nerve was to keep the response potential stable.
目的
探索一种合适的记录听神经和下丘单位放电的方法，以观察反应电位的特征。
方法
用玻璃微电极记录健康成年猫听神经和下丘对声刺激及蜗内电刺激的单单位放电。
结果
（1）识别出听神经初级纤维的自发放电。（2）听神经初级纤维自发放电的波形为双相电位，正脉冲宽度为0.5毫秒。（3）听神经的放电频率从每秒几次到100次不等。（4）下丘对高频纯音刺激的反应缺乏锁相。（5）下丘对蜗内低频电刺激的反应表现出锁相。
结论
成功记录了听神经的自发放电和听觉诱发反应以及下丘的纯音诱发反应和电诱发反应。然而，记录听神经电诱发反应存在的技术问题是保持反应电位稳定。
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