Sentenac S, Fernandez C, Thuillier A, Lechat P, Aymard G
Clinical Pharmacology and Drug Monitoring Unit, Pitie-Salpetriere Hospital, Paris, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 15;793(2):317-24. doi: 10.1016/s1570-0232(03)00333-7.
A new high-performance liquid chromatography assay was developed for the determination of tenofovir, a nucleotide analogue, in plasma. A solid-liquid extraction procedure was coupled with a reversed-phase HPLC system. The system requires a mobile phase containing Na(2)HPO(4) buffer, tetrabutylammonium hydrogen sulfate and acetonitrile for different elution through a C(18) column with UV detection. The method proved to be accurate, precise and linear between 10 and 4000 ng/ml. The method was applied to determine trough levels of tenofovir in 11 HIV-infected patients with virologic failure under multiple antiretroviral therapy. This method was also successfully applied to a pharmacokinetic study in an HIV infected patient with renal failure.
开发了一种新的高效液相色谱法，用于测定血浆中的核苷酸类似物替诺福韦。固液萃取程序与反相高效液相色谱系统联用。该系统需要一种流动相，其中含有磷酸氢二钠缓冲液、硫酸氢四丁铵和乙腈，以便通过C(18)柱进行不同的洗脱，并采用紫外检测。该方法在10至4000 ng/ml之间被证明是准确、精密且呈线性的。该方法被应用于测定11例接受多种抗逆转录病毒治疗但病毒学失败的HIV感染患者的替诺福韦谷浓度。该方法还成功应用于一名肾衰竭HIV感染患者的药代动力学研究。
