采用反相高效液相色谱法同时测定制剂中阿司匹林和5-单硝酸异山梨酯的含量。

Simultaneous determination of aspirin and isosorbide 5-mononitrate in formulation by reversed phase high pressure liquid chromatography.

作者信息

Gandhimathi M, Ravi T K, Abraham Annie, Thomas Renu

机构信息

Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, 395, Sarojini Naidu Street, 641 044, Tamil Nadu, Coimbatore, India.

出版信息

J Pharm Biomed Anal. 2003 Aug 21;32(6):1145-8. doi: 10.1016/s0731-7085(03)00248-6.

DOI:10.1016/s0731-7085(03)00248-6

PMID:12907257

Abstract

A simple, precise and rapid reversed phase HPLC method was developed for the simultaneous estimation of aspirin (AS) and isosorbide 5-mononitrate (ISM) in combined formulation. The method was carried out on a Thermo Quest C18 column using a mixture of water:methanol (water pH adjusted to 3.4 using dilute orthophosphoric acid) and detection was carried out at 215 nm using chlorzoxazone as internal standard. Both the drugs showed linearity in the range of 2-10 microg/ml and limits of quantification was found to be 4 and 40 ng/ml for AS and ISM, respectively.

摘要

建立了一种简单、精确且快速的反相高效液相色谱法，用于同时测定复方制剂中阿司匹林（AS）和5-单硝酸异山梨酯（ISM）的含量。该方法在Thermo Quest C18柱上进行，使用水：甲醇（用稀正磷酸将水的pH值调至3.4）的混合溶液，以氯唑沙宗为内标，在215 nm波长处进行检测。两种药物在2-10微克/毫升范围内均呈线性，AS和ISM的定量限分别为4和40纳克/毫升。